MAPPING OF THE PORCINE URATE OXIDASE AND TRANSFORMING GROWTH-FACTOR-BETA-2 GENES BY FLUORESCENCE IN-SITU HYBRIDIZATION

We have mapped two genes from human chromosome 1, urate oxidase (UOX) and transforming growth factor beta 2 (TGFB2), by fluorescence in situ hybridization (FISH) in the pig genome. Porcine-specific polymerase c hain reaction (PCR) primers for both genes were designed from the porc ine cDNA sequence. With the help of these primers yeast artificial chr omosome (YAC) clones for UOX and TGFB2 were isolated from a pig YAC li brary. These DNA probes were used for FISH analysis. TGFB2 was localiz ed to SSC 10p16. With the YAC probe for UOX two porcine chromosome reg ions 6q26 and 6q32, revealed specific signals. These results, help to refine the comparative mapping data between human and pig.