G. Rettenberger et al., MAPPING OF THE PORCINE URATE OXIDASE AND TRANSFORMING GROWTH-FACTOR-BETA-2 GENES BY FLUORESCENCE IN-SITU HYBRIDIZATION, Chromosome research, 4(2), 1996, pp. 147-150
We have mapped two genes from human chromosome 1, urate oxidase (UOX)
and transforming growth factor beta 2 (TGFB2), by fluorescence in situ
hybridization (FISH) in the pig genome. Porcine-specific polymerase c
hain reaction (PCR) primers for both genes were designed from the porc
ine cDNA sequence. With the help of these primers yeast artificial chr
omosome (YAC) clones for UOX and TGFB2 were isolated from a pig YAC li
brary. These DNA probes were used for FISH analysis. TGFB2 was localiz
ed to SSC 10p16. With the YAC probe for UOX two porcine chromosome reg
ions 6q26 and 6q32, revealed specific signals. These results, help to
refine the comparative mapping data between human and pig.