SERUM-PROTEIN ELECTROPHORESIS - GUIDELINE S FOR DIAGNOSIS EVALUATION IN THE DOG, CAT, AND HORSE

Zone electrophoresis at pH8,6 on cellulose acetate or on agarose gel i s the most commonly used method to separate serum proteins into fracti ons in order to study their repartition. The use of this technique is limited as it only allows a relatively imprecise fractionation into on ly five fractions (albumin, alpha 1-globulins, alpha 2-globulins, beta -globulins, gamma-globulins). Thus a specific diagnosis is seldom obta ined by this technique. But a thorough analysis of the electrophoresgr am and the alumin/globulins ratio provides a useful screening test in immune, renal, and hepatic disorders.