Jw. Kuluz et al., FRUCTOSE-1,6-BISPHOSPHATE REDUCES INFARCT VOLUME AFTER REVERSIBLE MIDDLE CEREBRAL-ARTERY OCCLUSION IN RATS, Stroke, 24(10), 1993, pp. 1576-1583
Background and Purpose: We tested the hypothesis that fructose-1,6-bis
phosphate, when administered 10 minutes before the end of 2 hours of r
eversible middle cerebral artery occlusion, reduces ischemia-reperfusi
on injury and infarct volume measured after a 3-day survival period in
rats. Methods: After 1 hour and 50 minutes of middle cerebral artery
occlusion by the intraluminal suture method, fructose-1,6-bisphosphate
, 500 mg/kg in group 1 and 350 mg/kg in group 2 (or an equivalent volu
me of 1.8% saline as placebo in each group), was given intravenously f
or a period of 15 minutes to fasted adult Sprague-Dawley rats. After 2
hours of ischemia, the suture was withdrawn and the rats allowed to s
urvive for 3 days. The areas of infarction in 10 hematoxylin-eosin-sta
ined coronal sections of the brain were measured and used to calculate
infarct volume. Results: In group 1, fructose-1,6-bisphosphate decrea
sed total cerebral hemispheric infarct volume by 43% (from 199.6+/-11.
2 to 114.2+/-35.8 mm3, P<.04; mean+/-SEM). Cerebral cortical and subco
rtical infarct volumes were decreased by 46% (from 137.3+/-7.5 to 74.1
+/-28.6 mm3, p<.04) and 36% (from 62.3+/-5.1 to 40.0+/-8.3 mm3, P<.04)
, respectively. In group 2, fructose-1,6-bisphosphate had no effect on
infarct volume in rats that developed mild intraischemic hyperthermia
, but in rats kept normothermic during ischemia, fructose-1,6-bisphosp
hate reduced subcortical infarct volume from 53.7+/-8.1 to 18.4+/-8.0
mm3 (P<.03). Conclusions: Fructose-1,6-bisphosphate improves functiona
l neurological outcome and reduces infarct volume after reversible mid
dle cerebral artery occlusion in rats.