Gr. Paterson et al., APPLICATION OF CAPILLARY ELECTROPHORESIS IN THE IDENTIFICATION OF PHENOTYPES CONTAINING THE BETA-LACTOGLOBULIN-C VARIANT, Journal of dairy science, 78(12), 1995, pp. 2637-2644
A method for the separation of the beta-LG variants A, B, and C was de
veloped using free zone capillary electrophoresis. A separation buffer
consisting of 50 mM 2-(N-morpholino)ethane sulfonic acid (pH 8.0) and
polyoxyethylene (20)-sorbitan monolaurate was used for the separation
. Milk samples from 424 Jersey cows were phenotyped using this method,
and the gene frequencies for beta-LG A, B, and C were .41, .53, and .
06, respectively. These frequencies were different from those found in
studies of Jersey populations in other countries, where the beta-LG B
allele had a significantly higher frequency, and the beta-LG A allele
a significantly lower frequency, than those of this present study. Th
e frequency of the beta-LG C allele was intermediate to those of other
studies. The concentration of beta-LG in the milk was different among
the beta-LG phenotypes and was, from greatest to least: AA, AB, AC, B
E, and BC.