Dj. Zack et al., 2 KAPPA-IMMUNOGLOBULIN LIGHT-CHAINS ARE SECRETED BY AN ANTI-DNA HYBRIDOMA - IMPLICATIONS FOR ISOTYPIC EXCLUSION, Molecular immunology, 32(17-18), 1995, pp. 1345-1353
An anti-DNA hybridoma derived from an MRL/lpr mouse secretes two diffe
rent kappa light chains in combination with a single heavy chain. Mult
iple single cell clones express and secrete immunoglobulin containing
both kappa tight chains. The N-terminal protein sequences of the light
chains correspond to sequences predicted from functionally rearranged
mRNAs subjected to reverse transcription and amplified by polymerase
chain reaction (PCR). Karyotype analysis of the hybridoma indicates a
clonal line derived from the fusion of two cells. By amino acid sequen
ce comparison and PCR analysis, both functional kappa light chains are
derived from the MRL/lpr spleen. The two functional light chain cDNAs
were cloned and co-transfected into COS-7 cells with the heavy chain
cDNA. Only one of the light chains in combination with mAb 3E10 heavy
chain confers anti-DNA reactivity. The presence of two separate kappa
light chains and, therefore, two separate antigen receptors on a singl
e B cell may have ramifications for both polyclonal activation and tol
eration of lupus B cells.