T. Matsumoto et al., ISOZYME VARIATION AND GENETIC RELATEDNESS AMONG NATURAL-POPULATIONS OF PLEUROTUS-OSTREATUS, Journal of General and Applied Microbiology, 41(6), 1995, pp. 487-497
The genetic relatedness among thirty-three natural isolates of an edib
le mushroom, Pleurotus ostreatus, collected from Japan, Europe, U.S.A.
and Korea was characterized by isoelectric focusing on polyacrylamide
gels. The activities of acid phosphatase, alcohol dehydrogenase, este
rase, laccase and malate dehydrogenase displayed 19, 9, 32, 11 and 10
distinct isozyme patterns, respectively. By combining the isozyme patt
erns obtained with the five enzymes, every isolate showed its own dist
inct electrophoretic phenotype. A distance matrix calculated between a
ll pairs of the 33 electrophoretic phenotypes based on the presence or
absence of isozyme bands was analyzed by the group-average method. Re
sults of the cluster analysis assigned the 33 phenotypes into four maj
or groups, each of which coincided with geographically distinct popula
tions. These results suggest that geographical distance between natura
l populations of P. ostreatus is correlated with the genetic divergenc
e.