VAS-DEFERENS ASPIRATION AND INTRACYTOPLASMIC INJECTION OF FROZEN-THAWED SPERMATOZOA IN A CASE OF ANEJACULATION IN A DIABETIC MAN

By using aspiration from the vas deferens, apparently good quality spe rmatozoa can be obtained for in-vitro fertilization (IVF) in cases of non-treatable anejaculation, Being squeezed from the epididymis during aspiration, the spermatozoa may be immature and their fertilizing cap acity lower than that of ejaculated spermatozoa, Our case report descr ibes a couple who achieved pregnancy when intracytoplasmic sperm injec tion (ICSI) was carried out with frozen-thawed spermatozoa aspirated f rom the vas deferens of a man whose anejaculation was associated with diabetes mellitus, In the aspiration, 50X10(6) spermatozoa were obtain ed, One half of them was frozen, and the other half was used fresh for conventional IVF, resulting in total fertilization failure of all the oocytes, The second treatment was ICSI, in which eight out of 11 oocy tes injected with frozen-thawed spermatozoa showed normal fertilizatio n, The second frozen embryo transfer resulted in a normal pregnancy.