I. Crandall et al., PLASMODIUM-FALCIPARUM - NATURALLY-OCCURRING RABBIT IMMUNOGLOBULINS RECOGNIZE HUMAN BAND-3 PEPTIDE MOTIFS AND MALARIA-INFECTED RED-CELLS, Experimental parasitology, 82(1), 1996, pp. 45-53
The reactivity to overlapping decapeptides based on sequences of human
band 3 protein was determined for 24 rabbit serum samples. A high per
centage of the sera recognized sequences of amino acid 650-670, as wel
l as several other sequences representing putative exofacial regions o
f the band 3 protein. When sera were used to stain immunofluorescently
human erythrocytes, some of which were infected with P. falciparum, t
hose samples that predominantly reacted with amino acids 650-670 stain
ed P. falciparum-infected red cells, whereas those that reacted with o
ther regions of band 3 stained all erythrocytes. A positive correlatio
n was found between anti-band 3 reactivity and the capacity of a serum
to inhibit the cytoadherence of P. falciparum-infected erythrocytes t
o C32 amelanotic melanoma cells. Also, some rabbit sera immunoprecipit
ated a high-molecular-weight protein from extracts of surface iodinate
d P. falciparum-infected red cells. (C) 1996 Academic Press, Inc.