EVALUATION OF 7 MICROSATELLITE LOCI IN SIMMENTAL CATTLE

Seven microsatellite loci were evaluated for their suitability for par entage control. The polymerase chain reaction (PCR) was used to amplif y the short tandem repeat (STR) loci in separate reactions. The micros atellite polymorphisms were visualized by radioisotopic autoradiograph ic detection. The microsatellite loci showed extensive polymorphism wi th allele numbers ranging from 4-23 and polymorphism information conte nt (PIC) values in the range of 0.57-0.87. The analysis of these loci also revealed that they have a 99.9% combined probability of exclusion (PE) of erroneous parentage. The results of this study revealed that a very high probability of exclusion could be reached with only four m icrosatellite loci.