SERUM-LIPID AND LIPOPROTEIN METABOLISM AFTER GLUCOSE-INGESTION IN NIDDM AND IGT PATIENTS - APOLIPOPROTEIN-CONTAINING PARTICLES

OBJECTIVE - Through analysis oi apolipoprotein-containing particles af ter glucose ingestion, we determined abnormal lipid metabolism in NIDD M patients and subjects with impaired glucose tolerance (IGT) without obesity or hyperlipidemia. RESEARCH DESIGN AND METHODS - Apolipoprotei n (ape) Al- or B100-containing particles or remnant-like particles (RL Ps) were isolated by immunoaffinity columns prepared with monoclonal a ntibodies. The cholesterol contents in these particles after glucose i ngestion (0, 10, 60, and 120 min) were analyzed in 18 nonobese NIDDM p atients, 18 nonobese IGT subjects, and 33 normal control subjects. RES ULTS - Changes in cholesterol content in apo B100 particles after oral glucose ingestion decreased significantly in both the NIDDM group and the IGT group; the changes were insignificant in the nondiabetic grou p. Changes in cholesterol content in the apo AI particles after oral g lucose ingestion showed no differences between the three groups. Chang es in cholesterol content in RLPs after oral glucose ingestion increas ed significantly in both the NIDDM group and IGT group; the changes we re insignificant in the nondiabetic group. The changes in apo B100 aft er oral glucose ingestion decreased significantly in the three groups; however, apo Al after oral glucose ingestion showed no change in the three groups. CONCLUSIONS - The cholesterol content in apo B100 partic les might decrease and the cholesterol content in RLPs might increase after oral glucose ingestion in not only NIDDM patients but also IGT s ubjects. The changes may be partially due to insulin resistance.