ON THE CYTOTOXICITY OF IRRADIATED MEDIA - TO WHAT EXTENT ARE STABLE PRODUCTS OF RADICAL-CHAIN REACTIONS IN PHYSIOLOGICAL SALINE RESPONSIBLEFOR CELL-DEATH
M. Saran et al., ON THE CYTOTOXICITY OF IRRADIATED MEDIA - TO WHAT EXTENT ARE STABLE PRODUCTS OF RADICAL-CHAIN REACTIONS IN PHYSIOLOGICAL SALINE RESPONSIBLEFOR CELL-DEATH, International journal of radiation biology, 64(3), 1993, pp. 311-318
Citations number
30
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
In a previous publication (Czapski et al. 1992) we reported that HOCl
accounts for the toxicity of irradiated phosphate-buffered saline towa
rds Escherichia coli bacterial cells. We have now investigated the res
pective toxicities towards lambda phage and mammalian cells. For phage
, as with bacteria, cytotoxicity of the irradiated media seems to deri
ve from HOCl without detectable contribution of H2O2. Mammalian cells
(V79 CHO), in contrast, are more sensitive to H2O2 than to HOCl. Both
agents, however, are not able to account quantitatively for the toxici
ty of irradiated solutions towards V79 cells; a hitherto unidentified
chlorine/oxygen derivative-being formed in the sub-micromolar concentr
ation range-is suggested to be responsible for toxicity in the case of
eukaryotes.