T7 DNA-DEPENDENT RNA-POLYMERASE CAN TRANSCRIBE RNA FROM TICK-BORNE ENCEPHALITIS-VIRUS (TBEV) CDNA WITH SP6 PROMOTER

Authors
DOBRIKOVA EY PLETNEV AG KARAMYSHEV VN MOROZOVA OV
Citation
Ey. Dobrikova et al., T7 DNA-DEPENDENT RNA-POLYMERASE CAN TRANSCRIBE RNA FROM TICK-BORNE ENCEPHALITIS-VIRUS (TBEV) CDNA WITH SP6 PROMOTER, FEBS letters, 382(3), 1996, pp. 327-329
Citations number
15
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
382
Issue
3
Year of publication
1996
Pages
327 - 329
Database
ISI
SICI code
0014-5793(1996)382:3<327:TDRCTR>2.0.ZU;2-F
Abstract
T7 RNA polymerase is shown to recognize the SP6 promoter including 17 base pairs before the transcription start site and produce the 5'-end TBEV RNA. The yield of TBEV RNA synthesized by heterologous T7 RNA pol ymerase from cDNA construction with SP6 promoter is higher than the RN A production by homologous SP6 RNA polymerase. The addition of 1 pmol template DNA with SP6 17 bp promoter in transcription mixture for SP6 or T7 RNA polymerases resulted in 1-5 X 10(-2) pmol RNA production.