S. Thurl et al., QUANTIFICATION OF INDIVIDUAL OLIGOSACCHARIDE COMPOUNDS FROM HUMAN-MILK USING HIGH-PH ANION-EXCHANGE CHROMATOGRAPHY, Analytical biochemistry, 235(2), 1996, pp. 202-206
A quantification method suitable for determination of individual oligo
saccharide compounds from human milk has been established. The crude m
ilk oligosaccharide fraction was separated into acidic oligosaccharide
s, neutral oligosaccharides, and lactose by gel permeation chromatogra
phy. After this separation step neutral and acidic oligosaccharides we
re analyzed separately by high-ps anion-exchange chromatography with p
ulsed amperometric detection, The concentrations of 14 neutral oligosa
ccharides, of 6 acidic oligosaccharides, and of N-acetylneuraminic aci
d were determined on the internal standards stachyose and galacturonic
acid, respectively, Thus, previously applied quantification methods f
or milk oligosaccharides based on gel permeation chromatography have b
een decisively improved. (C) 1996 Academic Press, Inc.