CONSEQUENCES OF NIGROSTRIATAL DENERVATION ON THE GAMMA-AMINOBUTYRIC ACIDIC NEURONS OF SUBSTANTIA-NIGRA PARS RETICULATA AND SUPERIOR COLLICULUS IN PARKINSONIAN SYNDROMES

To examine the effects of nigrostriatal denervation on the substantia nigra pars reticulata (SNpr), one of the main outputs of the basal gan glia, we used quantitative in situ hybridization to analyze the messen ger RNA coding for Mr 67,000 glutamic acid decarboxylase (GAD(67) mRNA ) in the SNpr neurons from patients with Parkinson's disease (PD), mon keys rendered parkinsonian by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyri dine (MPTP), and their respective controls. In MPTP-intoxicated monkey s, the expression of GAD(67) mRNA was increased in the SNpr neurons, a nd the increase was reversed by L-dopa treatment. There were no differ ences in the levels of GAD(67) mRNA between PD patients who had been t reated with L-dopa and control subjects. Combined with the previously reported increased expression of GAD(67) mRNA in the internal segment of the pallidum of MPTP-intoxicated monkeys, these data suggest that t he gamma-aminobutyric acidic (GABAergic) activity of the output system of the basal ganglia is globally increased by nigrostriatal denervati on. We also analyzed the level of GAD(67) mRNA expression in the super ior colliculus, a structure that receives the inhibitory influence of the GABAergic neurons of the SNpr and that is involved in eye movement control. GAD(67) mRNA expression was reduced in both MPTP-intoxicated monkeys, whether or not they received L-dopa therapy, and PD patients , compared to their respective controls. This decrease may result from the hyperactivity of the inhibitory nigrotectal pathway, but also fro m other influences since it was not corrected by L-dopa therapy. These changes may account for the slight ocular motor and visuospatial cogn itive impairment occurring in PD, even after L-dopa therapy.