THE FLUORESCENCE CHARACTERISTICS OF COMPL EXES OF NALIDIXIC-ACID WITHALKALI-EARTH METALS AND ITS APPLICATION

The fluorescence characteristics of complexes of nalidixic acid with a lkali earth metals (Mg, Ca, Sr and Ba) have been studied in this paper , It was found that in the pH 7 similar to 9 media the alkali earth me tals ions could form stable fluorescent complexes, Their fluorescence intensities enhanced with the decrease of ionic radii of alkali earth metals, Among them the fluorescence intensity of magnesium-nalidixic a cid system was 58 folds more than that of nalidixic acid itself, A new method for the determination of nalidixic acid using the first order derivative fluorescence excitation spectrum has been developed, The de tection limit was found to be 0.015 mu g/mL, This method has been used to determine nalidixic acid in human urine with recoveries of 96.6% s imilar to 102.6%.