ANALYSIS OF DNA FRAGMENTS AND PCR PRODUCT S FROM ONCOGENE USING CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION

Authors
REN JC DENG XY CAO Y YAO KT
Citation
Jc. Ren et al., ANALYSIS OF DNA FRAGMENTS AND PCR PRODUCT S FROM ONCOGENE USING CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE DETECTION, Gaodeng xuexiao huaxue xuebao, 17(3), 1996, pp. 362-366
Citations number
5
Categorie Soggetti
Chemistry
Journal title
Gaodeng xuexiao huaxue xuebao → ACNP
ISSN journal
02510790
Volume
17
Issue
3
Year of publication
1996
Pages
362 - 366
Database
ISI
SICI code
0251-0790(1996)17:3<362:AODFAP>2.0.ZU;2-Y
Abstract
Capillary electrophoresis-laser induced fluorescence detection system is applied to detect DNA fragments and PCR products from oncogene usin g hydroxypropylmethylcellulose and no-cross-linked polyacrylamide as s ieving media, The effects of sieving medium concentration, electrolyte concentration and intercalating agent concentration on separation of DNA fragments have been investigated in detail, The relationship betwe en peak area and concentration of DNA fragments in pressure injection has been compared with that of electrokinetic injection.