D. Nolte et al., THE HUMAN S3A RIBOSOMAL-PROTEIN - SEQUENCE, LOCATION AND CELL-FREE TRANSCRIPTION OF THE FUNCTIONAL GENE, Gene, 169(2), 1996, pp. 179-185
The intron-containing gene encoding human ribosomal protein S3a (hRPS3
a) was isolated by utilizing a PCR-based strategy to detect a gene-spe
cific intron which was subsequently used as a probe for cloning of the
entire gene. The hRPS3a gene is composed of six exons and five intron
s spanning 5013 bp. As described for other hRP-encoding genes, the pro
moter lacks a canonical TATA sequence and a defined CAAT box. Primer e
xtension experiments, as well as cell-free transcription, revealed tha
t a cytosine functions as the major transcription start point in a pol
ypyrimidine region, but a guanosine at position -1 was also able to in
itiate transcription. Hybridization analysis of chromosomal DNA from a
panel of human-rodent somatic cell hybrids revealed that hRPS3a is en
coded by a single locus in the human genome, present on chromosome 4.