QUANTITATION DETERMINATION OF HYPEROXIDE IN THE GLYCEROL SOLUTION OF BETULA-VERRUCOSA, BY THIN-LAYER

A method for quantitative determination of the hyperosid from glycerol solution of Betula Verrucosa, using TCL/phtodensitometry, is discusse d in detail. The sample was cleaned by solid phase extraction (C-18) a nd then applied on thin-layer plate. For TLC silica gel plates Sil G F -254 were used and as eluent: ethyl acetate/formic acid/water/(80:10:1 0,v/v). A standard solution of hyperosid was extracted on solid phase (C-18) and the results showed a very good recovery (99%). A regression equation for quantification hyperosid from glycerol solution of Betul a Verrucosa was used. The quantity of hyperosid was found to be 0.044 mg/mL in glycerol solution of Betula Verrucosa.