REHYBRIDIZATION ON METAPHASES STUDIED PREVIOUSLY BY FISH - AN APPROACH TO ANALYZE CHROMOSOME-ABERRATIONS

Authors
WANG MR PERISSEL B MALET P
Citation
Mr. Wang et al., REHYBRIDIZATION ON METAPHASES STUDIED PREVIOUSLY BY FISH - AN APPROACH TO ANALYZE CHROMOSOME-ABERRATIONS, Cancer genetics and cytogenetics, 85(1), 1995, pp. 58-60
Citations number
4
Categorie Soggetti
Oncology,"Genetics & Heredity
Journal title
Cancer genetics and cytogenetics → ACNP
ISSN journal
01654608
Volume
85
Issue
1
Year of publication
1995
Pages
58 - 60
Database
ISI
SICI code
0165-4608(1995)85:1<58:ROMSPB>2.0.ZU;2-7
Abstract
A new protocol for fluorescence in situ rehybridization is described. Biotin-labeled chromosome-specific DNA probes were hybridized onto met aphases which previously had been studied by FISH. This method makes i t possible to reexamine the same metaphase spreads with different DNA probes. It allows for precise characterization of cytogenetic transloc ations and for detection of multiple aberrations presented in a karyot ype. It is especially useful in cases where a limited number of cytoge netic preparations are available.