DIRECT RESOLUTION OF OPTICALLY-ACTIVE ISOMERS ON CHIRAL PACKINGS CONTAINING ERGOLINE SKELETONS .5. ENANTIOSEPARATION OF AMINO-ACID DERIVATIVES

A new procedure for ergot alkaloid-based chiral stationary phase prepa ration is described. Synthesis is based on bonding the allyl derivativ e of terguride to mercaptopropylsilanized silica gel. The packing exhi bits higher content of chiral selector, stability, reproducibility, an d enantioselectivity toward amino acids compared to that previously st udied. The chromatographic behavior of amino acids with different side chains and substituent groups is investigated in order to obtain a de eper insight into the enantiodiscriminative mechanism as well as to de termine the limitations and strengths of terguride as a chiral selecto r for this class of compounds. A variety of factors, including mobile phase parameters such as pH, ionic strength, content and nature of org anic modifier, and temperature, are examined.