Fc. Cochrane et al., APPLICATION OF TRIS(HYDROXYMETHYL)PHOSPHINE AS A COUPLING AGENT FOR ALCOHOL-DEHYDROGENASE IMMOBILIZATION, Enzyme and microbial technology, 18(5), 1996, pp. 373-378
The use of tris(hydroxymethyl)phosphine as a coupling reagent for the
immobilization of alcohol dehydrogenase onto chitosan films and for th
e attachment of the chitosan film to a glass support resulted in enzym
e activities far above those obtained by adsorption of enzyme, and gre
ater than those observed when using the more conventional glutaraldehy
de-coupling protocol. The stability of the chitosan films was dramatic
ally increased by covalent attachment to the glass using tris(hydroxym
ethyl)phosphine. Similar enzyme activities were found for coupling wit
h tris(hydroxymethyl)phosphine and glutaraldehyde on aminopropyl silic
a and aminopropyl glass. The tris(hydroxymethyl)phosphine coupling ext
ended the longevity of the alcohol dehydrogenase activity brit did not
alter the pH optima or K-m of the enzyme.