Microbial denitrification of drinking water was studied in laboratory
columns packed with shredded newspapers. Newspaper served as the sole
carbon and energy substrate as well as the only physical support for t
he microbial population. Complete removal of nitrate (100 mg 1(-1)) wa
s readily achieved, without accumulation of nitrite. The treated water
contained low dissolved organic carbon (4-10 mg 1(-1)). The cellulose
-dependent denitrification process was sensitive to changes in tempera
ture: nitrate removal rates at 14 degrees C were approximately one thi
rd of the rates observed at 32 degrees C. Pretreatment of newspaper wi
th diluted NaOH or diluted HCL, or by autoclave did not improve the ef
ficiency of the process. A time-dependent decay in denitrification rat
e was noticeable after several months of operation. The reasons for th
is phenomenon, which may be due to weakened adhesion of the bacteria t
o the substrate, are under investigation.