C. Elfakir et M. Dreux, SIMULTANEOUS ANALYSIS OF INTACT AND DESULFATED GLUCOSINOLATES WITH A POROUS GRAPHITIZED CARBON COLUMN, Journal of chromatography, 727(1), 1996, pp. 71-82
Citations number
26
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A porous graphitized column was evaluated for the qualitative analysis
of glucosinolates and desulfoglucosinolates. Successful separations w
ere achieved using 0.1% aqueous trifluoroacetic acid-acetonitrile (85:
15, v/v) as eluent. The selectivity obtained by LC on Hypercarb S allo
ws compounds with a slight hydrophobic structure difference (e.g., a d
ifference of one CH2 unit between two glucosinolates) to be satisfacto
rily resolved. Excellent separation of the two diastereoisomers progoi
trin and epiprogoitrin in anionic or neutral form was observed.