SIMULTANEOUS ANALYSIS OF INTACT AND DESULFATED GLUCOSINOLATES WITH A POROUS GRAPHITIZED CARBON COLUMN

A porous graphitized column was evaluated for the qualitative analysis of glucosinolates and desulfoglucosinolates. Successful separations w ere achieved using 0.1% aqueous trifluoroacetic acid-acetonitrile (85: 15, v/v) as eluent. The selectivity obtained by LC on Hypercarb S allo ws compounds with a slight hydrophobic structure difference (e.g., a d ifference of one CH2 unit between two glucosinolates) to be satisfacto rily resolved. Excellent separation of the two diastereoisomers progoi trin and epiprogoitrin in anionic or neutral form was observed.