GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF 2 NEWLY ESTABLISHED RENAL-CELL CARCINOMA CELL-LINES

Two new cell lines from human renal cell carcinoma are reported. Prima ry cell cultures from 75 consecutive cases of nephrectomy and metastat ic surgery due to different stages of RCC during 4 years were studied. Two cell cultures could be propagated for more than 50 passages in vi tro. HN4 was derived from a grade III clear cell carcinoma. HN51 origi nated from a metastatic brain lesion of a clear cell carcinoma grade I II. Karyotype analyses of HN4 revealed triploidy with a clonal aberrat ion, der(10)t(3;10)(q13;p12). HN51 also had a triploid pattern with di fferent marker chromosomes but without any clonal aberration. Loss of heterozygosity studies revealed no loss of heterozygosity on 3p or oth er chromosomal markers in HN4 but LOH was found on one 3p marker and o ne 14q marker in addition to all 17p and q markers in HN51. In vitro l ight microscopy showed distinctly different morphology in the two cell lines although they both had a typical epithelial growth pattern. Dou bling times in vitro were low but slightly higher for HN51. Repeated t umorigenenic experiments in athymic mice only gave rise to subcutaneou s tumors with HN51. On characterization by 2-dimensional gel electroph oresis, the two cell lines exhibited different polypeptide patterns wi th higher expression of proliferating cell nuclear antigen in HN51 and higher expression of glutathione-S-transferase in HN4 constituting th e most prominent differences.