CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST INFECTIOUS LARYNGOTRACHEITIS VIRUS

Nine monoclonal antibodies (MCAs) produced against two different strai ns of infectious laryngotracheitis virus (ILTV) were characterized and compared to previously characterized MCA 131-6, produced against: a t hird ILTV strain. In western blotting experiments, MCAs C, E, and 11 r esembled MCA 131-6, detecting proteins of 205, 160, 115, and 90 kD as well as several proteins less than 49 kD. The other six MCAs differed from previously described ILTV MCA. MCA D detected a 90-kD protein alo ng with several less than 49 kD. MCAs 4 and 5 each detected proteins o f 205, 160, 100, 90, and 70 kD. MCA 9 detected the same proteins detec ted by MCAs 4 and 5 except the 160-kD protein. MCA 10 detected protein s of 100, 90, and 70 kD and several proteins less than 43 kD. MCAs C, D, and E, like MCA 131-6, failed to react with any ILTV grown in the p resence of tunicamycin, suggesting that those MCAs are specific for ca rbohydrate-based epitopes. MCA 6 reacted with only a 100-kD protein in the presence or absence of tunicamycin. The remaining MCA detected on ly a 70-kD protein in the presence of tunicamycin except MCA 5, which reacted with proteins of 70 and 90 kD. Only MCA 4 and 6 neutralized IL TV infectivity.