PREPARATION AND ANALYSIS OF SOME ACETOSUGAR ESTERS OF ABSCISIC-ACID AND DERIVATIVES

Racemic abscisic acid (ABA), the cis and trans 1', 4'-diols (ABA diols ) derived from ABA by reduction of the 4' ketone, and the correspondin g 4'-O-acetates were converted into various acetosugar esters by react ion of their cesium salts with the 1-chloroacetosugars derived from gl ucose, galactose, lactose, and maltose. Analytical separations of the acetosugar esters using high-performance liquid chromatography (LC) on reverse-phase columns were developed. Continuous flow secondary ion m ass spectra (CFSIMS) of the various acetosugar eaters were obtained an d an LC/CFSIMS protocol employing multiple reaction monitoring was use d to detect ABA acetoglucose ester in an acetylated extract obtained f rom plant cells that had been treated with ABA.