For all water bodies especially for sea, lakes, and ground waters it i
s very important that nutrient pollution is the least possible (minima
l). From municipal and industrial waste waters where considerable amou
nt of nutrients is expected it is possible to remove nutrients with bi
ological treatment. The first step is usually aerobic activated sludge
treatment and the second step is combined anaerobic-anoxic-aerobic st
ep in different combinations. A very effective process for nitrogen re
moval is anoxic activated sludge system. It is very important that in
the first aerobic step complete nitrification is achieved. In our labo
ratory denitrification kinetics was studied. The objective of this stu
dy was determination of denitrification velocity and attendance of dif
ferent parallel phenomena such as nitrite, disappearance of nitrate an
d nitrite, sulphide formation, etc. Denitrification process was studie
d in two systems, in closed system (such as in dosed reactors for deni
trification) and in open systems (such as in open denitrification tank
s). We found out that denitrification rate in dosed systems was from a
bout 8 to 52 mg/l.h and was dependent on activated sludge type, sludge
concentration, and temperature of the system. In open system (such as
in an anoxic aeration tank) the rate of denitrification was lower tha
n in closed system; it was from 4 to 15 mg/l.h dependent on activated
sludge type, sludge concentration, and temperature of the system. Expe
riments were carried out in the range of temperature from 20 degrees t
o 30 degrees C.