EXPLORING THE FUNCTIONAL ROBUSTNESS OF AN ENZYME BY IN-VITRO EVOLUTION

The evolution of natural proteins is thought to have occurred by succe ssive fixation of individual mutations. In vitro protein evolution see ks to accelerate this process, RNA hypermutagenesis, cDNA synthesis in the presence of biased dNTP concentrations, delivers elevated mutant and mutation frequencies. Here lineages of active enzymes descended fr om the homotetrameric 78 residue dihydrofolate reductase (DHFR) encode d by the Escherichia coli R67 plasmid were generated by iterative RNA hypermutagenesis, resulting in >20% amino acid replacement, The 22 res idue N-terminus could be deleted yielding a minimum functional entity refractory to further changes, designating it as a determinant of R67 robustness, Complete substitution of the segment still allowed fixatio n of mutations, By the facile introduction of multiple mutations, RNA hypermutagenesis allows the generation of active proteins derived from extant genes through a mode unexplored by natural selection.