T-CELLS FROM BAX-ALPHA TRANSGENIC MICE SHOW ACCELERATED APOPTOSIS IN RESPONSE TO STIMULI BUT DO NOT SHOW RESTORED DNA DAMAGE-INDUCED CELL-DEATH IN THE ABSENCE OF P53
Hjm. Brady et al., T-CELLS FROM BAX-ALPHA TRANSGENIC MICE SHOW ACCELERATED APOPTOSIS IN RESPONSE TO STIMULI BUT DO NOT SHOW RESTORED DNA DAMAGE-INDUCED CELL-DEATH IN THE ABSENCE OF P53, EMBO journal, 15(6), 1996, pp. 1221-1230
Bax alpha was isolated due to its interaction with Bcl-2. Bax alpha ov
erexpression in an interleukin (IL)-3 dependent cell line accelerates
apoptosis upon removal of the cytokine, The ratio of Bax alpha to Bcl-
2 appears to be crucial for the effect, To study the action of the bax
gene product in vivo, we have generated transgenic mice overexpressin
g Bax alpha specifically in T cells. Such T cells show accelerated apo
ptosis in response to gamma-radiation, dexamethasone and etoposide, By
crossing bax alpha mice with bcl-2 transgenics we show that the criti
cal nature of the Bax alpha:Bcl-2 ratio holds in primary T cells and t
hat it can be manipulated to elicit a strong response to previously re
sisted stimuli, p53 has a role in the regulation of apoptosis in respo
nse to DNA-damaging agents. p53 directly activates transcription of th
e bax gene. The presence of the bax alpha transgene accelerated apopto
sis in thymocytes from both p53-/- and p53+/- mice in response to dexa
methasone. Thymocytes from p53-/- mice with the bax alpha transgene sh
owed similar resistance to apoptosis by DNA-damaging agents as did p53
-/- mice without the transgene. Bax alpha overexpression alone cannot
restore the DNA damage apoptosis pathway, suggesting that p53 is requi
red to induce or activate other factor(s) to reconstitute the response
fully.