USE OF ENZYME SOMASE(R) THE MEASUREMENT OF BIOFILM USING BIOLUMINESCENCE

Authors
GREETHAM M HOLDEN B SCUTT J
Citation
M. Greetham et al., USE OF ENZYME SOMASE(R) THE MEASUREMENT OF BIOFILM USING BIOLUMINESCENCE, Water science and technology, 32(8), 1995, pp. 221-225
Citations number
7
Categorie Soggetti
Water Resources","Environmental Sciences","Engineering, Civil
Journal title
Water science and technology → ACNP
ISSN journal
02731223
Volume
32
Issue
8
Year of publication
1995
Pages
221 - 225
Database
ISI
SICI code
0273-1223(1995)32:8<221:UOESTM>2.0.ZU;2-O
Abstract
Analysis of the biofilm on a distribution system model pipe rig gave h igh readings of extracellular adenosine-5'-triphosphate (ATP). The fre e ATP was assumed to come from dead cells at the base of the biofilm. The levels were large in proportion to the measured cellular ATP and w ere unstable. This resulted in errors in the calculation of cellular A TP. An enzyme Somase (0.5%) was employed to hydrolyse the free ATP. In creased accuracy of the measurement of cellular ATF was seen.