REGULATION, EXPRESSION AND FUNCTION OF A NEW BASIC CHITINASE GENE IN RICE (ORYZA-SATIVA L)

A new basic chitinase gene, designated RC24, was isolated from a rice genomic library. The predicted RC24 protein contains 322 amino acid re sidues and exhibits 68% to 95%, amino acid identity with known class I rice chitinases. RC24 protein expressed in Escherichia coli exhibited chitinase activity and strongly inhibited bacterial growth. Two trans cription start sites of the RC24 gene were mapped by primer extension analysis of both rice native RNA and in vitro transcribed RNA using a RC24 promoter/GUS (beta-glucuronidase) gene fusion as a template. The 5'-franking region of RC24 contained several putative stress-responsiv e cis-acting elements. A basal level of RC24 transcripts was detected in rice root and stem tissues, but not in leaf tissues. RC24 transcrip ts rapidly accumulated within 1 h after fungal elicitor treatment of s uspension-cultured cells, and the levels continued to increase for at least 9 h. RC24 transcript accumulation was also observed in intact le af tissues upon wounding. Transgenic rice plants containing the RC24/G US gene fusion further confirmed that the RC24 gene showed a tissue-sp ecific expression pattern and that transcription of the RC24 promoter was sensitively and rapidly activated by wounding.