Dh. Bringloe et al., DIFFERENTIAL GENE-EXPRESSION OF CHLOROPLAST AND CYTOSOLIC PHOSPHOGLYCERATE KINASE IN TOBACCO, Plant molecular biology, 30(3), 1996, pp. 637-640
Northern blot analysis of RNA extracted from leaves of increasing age
and different organs, indicates that genes encoding both isoenzymes of
tobacco phosphoglycerate kinase (PGK, EC 2.7.2.3) are differentially
expressed in a developmental and tissue-specific manner. The genes for
both chloroplast PGK (chl-PGK) and cytosolic PGK (cyt-PGK) also show
light-modulated gene expression in vivo. In dark-grown developing coty
ledonary leaves of tobacco both PGK mRNAs are present, but only the co
ncentration of the chl-PGK mRNA increased on illumination. In contrast
, on transfer to darkness, the concentration of both mRNAs decreased i
n light-grown seedlings and then increased again on resumption of illu
mination.