Conventional methods have been used to study the general configuration
of the wall of the Sinus paranalis in dogs. Apocrine tubular glands,
elastic fibres and smooth-muscle fibres are the more significant eleme
nts of the wall, together with the epithelium and connective tissue. B
y means of the immunohistochemical method described in this paper, the
results provide histochemical evidence for the presence of an AChE-po
sitive reaction, and supposedly cholinergic nerve fibres in the wall o
f the dog anal sacs, mainly associated with subepithelial smooth muscl
e, vessels, and glands.