Jr. Reddan et al., REGIONAL DIFFERENCES IN THE DISTRIBUTION OF CATALASE IN THE EPITHELIUM OF THE OCULAR LENS, Cellular and molecular biology, 42(2), 1996, pp. 209-219
Oxidative stress is thought to play a major role in cataract formation
. The present experiments are aimed at gaining a better understanding
of the systems that protect the lens from damage by reactive oxygen sp
ecies. The aqueous humor normally contains hydrogen peroxide (H2O2), a
compound capable of generating reactive oxygen species. The systems p
rotecting the ocular lens from oxidative damage are primarily confined
to the epithelium, a single layer of cells on the anterior side of th
e organ directly beneath the lens capsule. When cultured rabbit lenses
were challenged with a single dose of 0.2 mM H2O2, cells in the perip
heral region of the epithelium survived; those in the central region d
ied. Here we investigate the histochemical and immunoperoxidase distri
butions of catalase, an enzyme which detoxifies H2O2, in cells from th
e peripheral and central regions of the epithelium on flat mount prepa
rations of the epithelium. In a flat mount, the entire population of l
ens epithelial cells can be viewed on one preparation. The reaction pr
oduct for catalase activity and its immunoperoxidase localization were
more intense in peripheral epithelial cells than in cells throughout
the central epithelium. Treatment of cultured lens epithelial cells or
rabbit lenses with 3-aminotriazole or potassium cyanide, inhibitors o
f catalase, reduced or abolished the histochemical reaction product. U
ltrastructural cytochemistry confirmed the presence of catalase in mic
roperoxisomes of the epithelial cells from whole lenses. The decreased
level of catalase throughout the central epithelium may account for t
he increased susceptibility of these cells to H2O2-induced cell death.