M. Nagae et al., MONOCLONAL-ANTIBODY AGAINST AN AMNIOTIC PROTEIN CARRYING ABH BLOOD-GROUP EPITOPES AND ITS FORENSIC APPLICATION, Tohoku Journal of Experimental Medicine, 177(4), 1995, pp. 353-364
A mouse monoclonal antibody against an amniotic protein carrying ABH a
ntigenic epitopes was established. BALB/c mice were immunized by an am
niotic protein of molecular weight over 200 kDa, which had proved to b
e the carrier protein of ABH blood group epitopes by analysis with SDS
-PAGE and immunoblotting. The antibody, ASP-1, was directed to the amn
iotic carrier protein without affecting the ABH blood group antigenici
ty, and did not cross-react with other body fluids which included bloo
d, saliva, semen, urine or vaginal secretion. The immunoglobulin class
of ASP-1 was IgG1 with a titer of 1 : 1,600. ASP-1 was used to detect
the ABH blood group of amniotic fluid by the sandwich ELISA in which
wells of plates were coated with ASP-1, and the ABH blood group of the
captured protein was detected with mouse IgM anti-ii and -B antibodie
s and enzyme conjugated anti-mouse IgM. The sandwich ELISA could succe
ssfully detect the blood group of amniotic fluid in mixed body fluids.