MONOCLONAL-ANTIBODY AGAINST AN AMNIOTIC PROTEIN CARRYING ABH BLOOD-GROUP EPITOPES AND ITS FORENSIC APPLICATION

A mouse monoclonal antibody against an amniotic protein carrying ABH a ntigenic epitopes was established. BALB/c mice were immunized by an am niotic protein of molecular weight over 200 kDa, which had proved to b e the carrier protein of ABH blood group epitopes by analysis with SDS -PAGE and immunoblotting. The antibody, ASP-1, was directed to the amn iotic carrier protein without affecting the ABH blood group antigenici ty, and did not cross-react with other body fluids which included bloo d, saliva, semen, urine or vaginal secretion. The immunoglobulin class of ASP-1 was IgG1 with a titer of 1 : 1,600. ASP-1 was used to detect the ABH blood group of amniotic fluid by the sandwich ELISA in which wells of plates were coated with ASP-1, and the ABH blood group of the captured protein was detected with mouse IgM anti-ii and -B antibodie s and enzyme conjugated anti-mouse IgM. The sandwich ELISA could succe ssfully detect the blood group of amniotic fluid in mixed body fluids.