CHEMILUMINESCENCE SENSOR WITH MN(III)-TETRAKIS (4-SULFONATOPHENYL)-PORPHYRIN IMMOBILIZED ON DIOCTADECYLDIMETHYLAMMONIUM CHLORIDE BILAYER-MEMBRANES INCORPORATED INTO PVC FILM

An indicator phase for a flow-through chemiluminescence (CL) sensor co mposed of ordered surfactant assemblies, a polymer sind a catalyst was evaluated by measuring adrenaline. The method is based on use of Mn ( III)-porphyrin immobilized on a bilayer membrane contained in a blende d film, prepared by incorporating dioctadecyl-dimethylammonium chlorid e into polyvinyl chloride. The sensor consisted of a Pyrex glass tube (30 mm x 5 mm i.d.) packed with silica glass wool, on which the indica tor phase was coated, and a photomultiplier tube. The blend film funct ioned as a favorable reaction medium for the adrenaline CL, and furthe r enhanced CL was observed with the immobilized catalyst. This indicat or phase permitted adrenaline to be detected down to 3 x 10(-6) M with a 20 mu l injection into a 0.3 M NaOH carrier solution. The relative standard deviation (n = 10) was 1.0% for 5 x 10(-5) RI adrenaline. For 80 successive injections of 5 x 10(-5) M adrenaline, the variation of the CL signal was within the relative standard deviation. Almost the same sensitivity and precision were observed with the indicator phase stored in water for at least 3 days. The sensor was successfully appli ed to determine adrenaline in drug samples.