Je. Kountourellis et Ko. Ebete, DETERMINATION OF PIMETHIXENE IN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Analytical letters, 29(5), 1996, pp. 775-784
A high performance liquid chromatographic (HPLC) assay was developed f
or the determination of pimethixene in plasma and urine. Pimethixene w
as extracted by a rapid and simple liquid-liquid procedure using n-pen
tane:isopropyl alcohol (50:1), and a reversed phase HPLC separation te
chnique with UV spectrophotometric detection at 254 nm. Determination
was possible for pimethixene in the concentration range 53-530 ng/ml w
ith the limit of detection at 20 ng/ml for both plasma and urine. The
overall recoveries of pimethixene added to plasma and urine samples by
an external standard method were 95.6 and 98.5%, with coefficients of
variation of 0.9993 and 0.9997, respectively.