SIGMA(54)-DEPENDENT TRANSCRIPTION OF THE PSEUDOMONAS-PUTIDA XYLS OPERON IS INFLUENCED BY THE IIA(NTR) PROTEIN OF THE PHOSPHOTRANSFERASE SYSTEM IN ESCHERICHIA-COLI

Authors
DU Y HOLTEL A REIZER J SAIER MH
Citation
Y. Du et al., SIGMA(54)-DEPENDENT TRANSCRIPTION OF THE PSEUDOMONAS-PUTIDA XYLS OPERON IS INFLUENCED BY THE IIA(NTR) PROTEIN OF THE PHOSPHOTRANSFERASE SYSTEM IN ESCHERICHIA-COLI, Research in microbiology, 147(3), 1996, pp. 129-132
Citations number
10
Categorie Soggetti
Microbiology
Journal title
Research in microbiology → ACNP
ISSN journal
09232508
Volume
147
Issue
3
Year of publication
1996
Pages
129 - 132
Database
ISI
SICI code
0923-2508(1996)147:3<129:STOTPX>2.0.ZU;2-I
Abstract
IIA(Ntr) encoded within the rpoN operons of many Gram-negative bacteri a, is a homologue of a class of phosphoryl transfer proteins of the ph osphoenolpyruvate: sugar phosphotransferase system. We have used a xyl S operon-lacZ fusion from the TOL plasmid of Pseudomonas putida to sho w that IIA(Ntr) influences sigma(54)-dependent transcription when the xylS operon is expressed in Escherichia coli. Loss of IIA(Ntr) influen ces, but does not abolish cyclic AMP-independent carbon catabolite rep ression.