EFFECT OF THIOSULFATE AS ELECTRON-ACCEPTOR ON GLUCOSE AND XYLOSE OXIDATION BY THERMOANAEROBACTER-FINNII AND A THERMOANAEROBACTER SP ISOLATED FROM OIL-FIELD WATER

During glucose and xylose fermentation, Thermoanaerobacter finnii was observed to produce lactate, acetate, H-2 and CO2, with ethanol being the major end product. Thermoanaerobacter strain SEBR 5268, an isolate from an oil field, also produced a similar range of end products from glucose and xylose fermentation, with the exception that both ethanol and lactate were the major products of sugar metabolism. Both these s trains were able to reduce thiosulphate to sulphide in the presence of these two substrates, with acetate being the dominant metabolite in t hat case, In addition, a faster growth rate and increased cell yield w ere obtained in the presence of thiosulphate, than in its absence. The higher concentrations of acetate produced in the presence of thiosulp hate rather than without any electron acceptor indicated that more ATP was generated from substrate-level phosphorylation. These results hav e implications for our understanding of the breakdown of carbohydrates present in organic matter found in the natural ecological niches of T hermoanaerobacter species (sulphide-, elemental sulphur- or sulphate-r ich thermal hot springs and oil fields).