CONTRIBUTIONS TO STRUCTURAL AND DYNAMIC S TUDIES IN LIQUID-STATE NMR USING OFF-RESONANCE RF IRRADIATION

During the last few years, several experiments using off-resonance rf irradiation have been described for structural and dynamic studies of biomolecules in liquid state NMR. First, a concise description of the calculation leading to the expression of relaxation rates and a summar y of the intrinsic advantages of such methods are given. The resulting off-resonance ROESY sequence enables the structural study of ail kind s of molecules. The problem of internal dynamics is then discussed, fo cussing on the interest of using off-resonance rf fields. Several expe riments using them are described and compared with other procedures. F inally the trends of NMR development concerning the study of internal dynamics are mentioned.