INSIGHT INTO THE DISTRIBUTION OF MOLECULAR-WEIGHTS AND HIGHER-ORDER STRUCTURE OF HYALURONANS AND SOME BETA-(1-]3)-GLUCANS BY SIZE-EXCLUSIONCHROMATOGRAPHY

Authors
SOLTES L MISLOVICOVA D SEBILLE B
Citation
L. Soltes et al., INSIGHT INTO THE DISTRIBUTION OF MOLECULAR-WEIGHTS AND HIGHER-ORDER STRUCTURE OF HYALURONANS AND SOME BETA-(1-]3)-GLUCANS BY SIZE-EXCLUSIONCHROMATOGRAPHY, BMC. Biomedical chromatography, 10(2), 1996, pp. 53-59
Citations number
55
Categorie Soggetti
Chemistry Analytical","Pharmacology & Pharmacy",Biology,"Biochemical Research Methods
Journal title
BMC. Biomedical chromatography → ACNP
ISSN journal
02693879
Volume
10
Issue
2
Year of publication
1996
Pages
53 - 59
Database
ISI
SICI code
0269-3879(1996)10:2<53:IITDOM>2.0.ZU;2-6
Abstract
The effects of high energy ultrasound and slightly raised temperature combined with the denaturing action of dimethylsulphoxide on the molec ular weight and higher-order structure of hyaluronans and some beta-(1 -->, 3)-glucans were studied by means of size exclusion chromatography (SEC) technique. Some experimental conditions connected with the (bio -)polymer sample preparation prior to its SEC analysis are overviewed in the light of informational relevance of studies where the action of a physical and/or chemical agent changes the hydrodynamic size of the m omolecule.