W. Lesniak et A. Filipek, CA2-DEPENDENT INTERACTION OF CALCYCLIN WITH MEMBRANE(), Biochemical and biophysical research communications, 220(2), 1996, pp. 269-273
The presence of calcyclin in the microsomal fraction of Ehrlich ascite
s tumor cells was detected using polyclonal antibodies. Association of
calcyclin with the microsomes depended on the presence of calcium ion
s in the buffer used for cell fractionation. The interaction of calcyc
lin with Elhrlich ascites tumor cells microsomes was confirmed in the
in vitro conditions by cosedimentation assay using exogenous calcyclin
. It was shown that phospholipids extracted from natural membranes and
purified phosphatydylserine or phosphatydylcholine were not involved
in the binding. Instead, several low molecular weight polypeptides in
the Triton X-100 resistant membrane fraction were found to interact wi
th calcyclin. (C) 1996 Academic Press, Inc.