A NUCLEIC-ACID SEQUENCE-BASED AMPLIFICATION (NASBA) SYSTEM FOR LISTERIA-MONOCYTOGENES AND SIMPLE METHOD FOR DETECTION OF AMPLIMERS

Authors
BLAIS BW TURNER G SOOKNANAN R MALEK LT PHILLIPPE LM
Citation
Bw. Blais et al., A NUCLEIC-ACID SEQUENCE-BASED AMPLIFICATION (NASBA) SYSTEM FOR LISTERIA-MONOCYTOGENES AND SIMPLE METHOD FOR DETECTION OF AMPLIMERS, Biotechnology techniques, 10(3), 1996, pp. 189-194
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
3
Year of publication
1996
Pages
189 - 194
Database
ISI
SICI code
0951-208X(1996)10:3<189:ANSA(S>2.0.ZU;2-M
Abstract
A NASBA system amplifying specific sequences of the Listeria monocytog enes hlyA gene and an immunoenzymatic assay for the detection of ampli mers was developed. The immunoenzymatic assay utilized a simple microt iter plate format and an anti-RNA:DNA hybrid antibody for the detectio n of NASBA product (predominantly RNA) hybridized to an immobilized DN A probe. This highly sensitive isothermal amplification and detection system was reactive with genomic DNA from various L. monocytogenes iso lates but not with other Listeria or non-Listeria species.