FUNGAL PECTIC ENZYME FRACTIONATION BY DYE AFFINITY-CHROMATOGRAPHY

Authors
CAMPERI SA GRASSELLI M CASCONE O
Citation
Sa. Camperi et al., FUNGAL PECTIC ENZYME FRACTIONATION BY DYE AFFINITY-CHROMATOGRAPHY, Biotechnology techniques, 10(3), 1996, pp. 211-214
Citations number
17
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
10
Issue
3
Year of publication
1996
Pages
211 - 214
Database
ISI
SICI code
0951-208X(1996)10:3<211:FPEFBD>2.0.ZU;2-Y
Abstract
A dye affinity fractionation of a commercial pectic enzyme preparation is described. From nine immobilized triazinic dyes assayed, only Blue R-HE and Yellow 4R-HE were able to retain pectin lyase thus allowing the obtention of a fraction not producing methanol during fruit juice clarification. While pectinesterase did not bind to the chromatographi c matrix, the retained pectin lyase could be quantitatively eluted wit h a salt gradient.