EXPRESSION OF SMALL SYNTHETIC GENES-CODING FOR HEGF, HUMAN EPIDERMAL GROWTH-FACTOR, AND CPTI-II, SERINE PROTEINASE-INHIBITOR FROM CUCURBITACEA, CLONED IN A NOVEL EXPRESSION SECRETION VECTOR IN SACCHAROMYCES-CEREVISIAE/

Efficient synthesis of two small eukaryotic polypeptides of human and plant origin was carried out using a novel expression/secretion yeast vector, pYET. The yield was optimized in respect of the yeast strain, expression cassette construction, promoter regulation and culture cond itions. Both cloned genes code for biotechnologically important protei ns: human epidermal growth factor and a serine proteinase inhibitor fr om Cucurbitacea.