EXPRESSION OF SMALL SYNTHETIC GENES-CODING FOR HEGF, HUMAN EPIDERMAL GROWTH-FACTOR, AND CPTI-II, SERINE PROTEINASE-INHIBITOR FROM CUCURBITACEA, CLONED IN A NOVEL EXPRESSION SECRETION VECTOR IN SACCHAROMYCES-CEREVISIAE/
J. Topczewska et al., EXPRESSION OF SMALL SYNTHETIC GENES-CODING FOR HEGF, HUMAN EPIDERMAL GROWTH-FACTOR, AND CPTI-II, SERINE PROTEINASE-INHIBITOR FROM CUCURBITACEA, CLONED IN A NOVEL EXPRESSION SECRETION VECTOR IN SACCHAROMYCES-CEREVISIAE/, Acta Biochimica Polonica, 43(1), 1996, pp. 255-264
Efficient synthesis of two small eukaryotic polypeptides of human and
plant origin was carried out using a novel expression/secretion yeast
vector, pYET. The yield was optimized in respect of the yeast strain,
expression cassette construction, promoter regulation and culture cond
itions. Both cloned genes code for biotechnologically important protei
ns: human epidermal growth factor and a serine proteinase inhibitor fr
om Cucurbitacea.