LOW INFECTION FREQUENCY OF MACROPHAGES IN THE SPLEENS OF HIV+ PATIENTS

Macrophages are often considered as a reservoir of latent infection in HIV+ patients, and their infection may indeed be very important funct ionally. However, some quantitative studies did not find high infectio n frequencies in peripheral blood monocytes. Since lymphoid organs are the major site of infection, macrophage infection was tested in splee ns removed from HIV+ patients for treatment of different syndromes. Te n replicates of limiting dilutions from different cell populations wer e submitted to a nested PCR specific to conserved regions of HIV1 env DNA. On an average, 1/2,300 adherent cells carried HIV1 DNA (n = 7; ra nge: 1/55,000 to 1/660). These adherent cells, obtained after two days of culture, comprised the whole macrophage population, with no biases introduced by surface molecule selection, but were not pure (41-78% m acrophages). Only 1/37,000 CD14(+) monocyte/macrophages were positive (n = 6; range: 1/130,000 to 1/22,000). Therefore, the infection freque ncy of the isolated splenic monocytes/macrophages from these patients could be estimated at between 1/37,000 and 1/2,300. In contrast, 1/60 CD4(+) T lymphocytes were positive (n = 7; range: 1/190 to 1/17). With in the experimental limits, such as cell isolation, required for accur ate quantification, this study in the spleen indicates, as have other studies on peripheral blood, that macrophages do not quantitatively co nstitute an important reservoir of HIV when compared to CD4(+) T lymph ocytes.