AN IMPROVED METHOD FOR THE DETECTION OF TOSPOVIRUSES USING THE POLYMERASE CHAIN-REACTION

Authors
MUMFORD RA BARKER I WOOD KR
Citation
Ra. Mumford et al., AN IMPROVED METHOD FOR THE DETECTION OF TOSPOVIRUSES USING THE POLYMERASE CHAIN-REACTION, Journal of virological methods, 57(1), 1996, pp. 109-115
Citations number
16
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Journal title
Journal of virological methods → ACNP
ISSN journal
01660934
Volume
57
Issue
1
Year of publication
1996
Pages
109 - 115
Database
ISI
SICI code
0166-0934(1996)57:1<109:AIMFTD>2.0.ZU;2-P
Abstract
A reverse transcription-polymerase chain reaction (RT-PCR)-based assay for the detection of tomato spotted wilt virus (TSWV) has been improv ed and extended to enable the detection of additional Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (INSV) and another which detects all Tospoviruses tested, i ncluding TSWV, INSV, tomato chlorotic spot virus and groundnut ringspo t virus. An improved, rapid RNA extraction method is also described.