Ra. Mumford et al., AN IMPROVED METHOD FOR THE DETECTION OF TOSPOVIRUSES USING THE POLYMERASE CHAIN-REACTION, Journal of virological methods, 57(1), 1996, pp. 109-115
Citations number
16
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
A reverse transcription-polymerase chain reaction (RT-PCR)-based assay
for the detection of tomato spotted wilt virus (TSWV) has been improv
ed and extended to enable the detection of additional Tospoviruses. In
addition to TSWV-specific primers, two further pairs of primers have
been designed, one pair which specifically detects impatiens necrotic
spot virus (INSV) and another which detects all Tospoviruses tested, i
ncluding TSWV, INSV, tomato chlorotic spot virus and groundnut ringspo
t virus. An improved, rapid RNA extraction method is also described.