CLUSTER-ANALYSIS BY MEASUREMENT OF PEROXIDASE AND ESTERASE FROM CITRUS-FLAVEDO

The zymograms of peroxidase (POD) and esterase (EST) from seventy-two kinds of Citrus fruits and the fruit of both Poncirus and Fortunella w ere obtained by polyacrylamide gel electrophoresis. It was found that there was little variance in the band pattern of POD and EST of the fr uits harvested after September. There were at least three bands of POD and approximately ten of EST in each cultivar. The density of each ba nd for EST was measured with a densitometer. There was a characteristi c band for POD identifying pummelos. The band patterns of EST were als o characteristic among each species or cultivar. Two kinds of ootachib ana (Citrus otachibana Hort. ex Tanaka) were shown to be different cul tivars from each other according to their EST zymograms and peel oil a nalysis. Cluster analysis based on EST was done and discussed together with POD.