THYMINE DIMER-DEPENDENT INCISION ON ULTRAVIOLET-LIGHT DAMAGED-DNA IN CELL-FREE-EXTRACTS OF CHLORELLA-PYRENOIDOSA

Authors
CHANG CW HO JC HSU T
Citation
Cw. Chang et al., THYMINE DIMER-DEPENDENT INCISION ON ULTRAVIOLET-LIGHT DAMAGED-DNA IN CELL-FREE-EXTRACTS OF CHLORELLA-PYRENOIDOSA, Bioscience, biotechnology, and biochemistry, 60(3), 1996, pp. 490-492
Citations number
17
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
60
Issue
3
Year of publication
1996
Pages
490 - 492
Database
ISI
SICI code
0916-8451(1996)60:3<490:TDIOUD>2.0.ZU;2-B
Abstract
Cell-free extracts of the unicellular algae Chlorella pyrenoidosa indu ce a UV-dependent incision of both phi x 174 RFI DNA and a 27-mer doub le-stranded oligonucleotide containing a single thymine dimer, as anal yzed by agarose and denaturing polyacrylamide gel electrophoresis, res pectively. The incision reaction occurred only on the thymine dimer-co ntaining strand of the 27-mer, and a major incision product of 17-mer was observed. Addition of ATP or ATP-regenerating system to algal extr acts increased the effect of DNA incision.