Cw. Chang et al., THYMINE DIMER-DEPENDENT INCISION ON ULTRAVIOLET-LIGHT DAMAGED-DNA IN CELL-FREE-EXTRACTS OF CHLORELLA-PYRENOIDOSA, Bioscience, biotechnology, and biochemistry, 60(3), 1996, pp. 490-492
Cell-free extracts of the unicellular algae Chlorella pyrenoidosa indu
ce a UV-dependent incision of both phi x 174 RFI DNA and a 27-mer doub
le-stranded oligonucleotide containing a single thymine dimer, as anal
yzed by agarose and denaturing polyacrylamide gel electrophoresis, res
pectively. The incision reaction occurred only on the thymine dimer-co
ntaining strand of the 27-mer, and a major incision product of 17-mer
was observed. Addition of ATP or ATP-regenerating system to algal extr
acts increased the effect of DNA incision.