2 FORMS OF ALPHA-GLUCOSIDASE FROM MUCOR JAVANICUS PRODUCED BY CULTUREWITH CASTANOSPERMINE

When Mucorjavanicus was cultured with castanospermine, two a-glucosida ses (CS-I, II) were produced. The two enzymes were purified by two CM- cellulofine column chromatographies, and compared with the alpha-gluco sidase (C) from a culture without castanospermine. The molecular weigh ts of the three enzymes were calculated to be 97000 (CS-I, II) and 940 00 (C) from SDS-PAGE. CS-II was similar in hydrolysis of alpha-1,4-lin kage to C, but hydrolyzed alpha-1,2-, alpha-1,3-, and alpha-1,6-linkag es at a lower rate than C. The Michaelis constant of CS-I!for malto-ol igosaccharides was several times as high as the constants of CS-II and C, but CS-I hydrolyzed nigerose at a faster rate than maltose.